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For metabolic analysis of cultured cancer cells and tumours, frozen tumour tissue was homogenized in 80% methanol containing 20 mM NEM using a Precellys cold tissue homogenizer (Bertin) at a ratio of 20 mg tissue to 1 ml solvent

People just aren't thinking about the broader potential," Field noted

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Practical Application Omega-3 rich fish: Salmon, mackerel, sardines, anchovies Monounsaturated fats: Extra virgin olive oil, avocados, almonds Plant-based omega-3s: Flaxseeds, chia seeds, walnuts Medium-chain triglycerides: Coconut oil (moderate amounts) Include healthy fats in each meal (10-15 grams per meal) Pair fats with protein and fiber for sustained L-cell activation Avoid excessive saturated fat which may impair L-cell function Strategy 5: Meal Composition and Sequencing The Science: L-cells dont just respond to individual nutrientsthey respond to the pattern, sequence, and combination in which nutrients arrive