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As authors highlighted, inflammatory signaling may lead to inhibition of ETC activity, through induction of changes in the phosphorylation state of proteins and the reduction of the mitochondrial membrane potential

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For reducing SDS-PAGE, 2 g of protein was mixed with 3 L of 4 Bolt LDS Sample Buffer (Invitrogen), 1.2 L of 10 Bolt Sample Reducing Agent (Invitrogen), and Milli-Q water to a total volume of 12 L, and the samples were incubated for 10 min at 95 C

3 Results 3.1 Case series During the study period, thirty-nine patients experienced new-onset AP while using GLP-1 RAs (Table 1)