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Hormone replacement therapy (HRT) is a category of prescription treatment used to support relief from menopausal symptoms in eligible patients

However, these same pathways might also intensify craving during initial withdrawal phases, potentially accelerating relapse

Membranes were sequentially incubated with primary antibodies (anti-GLP-1 [Santa Cruz, sc-80603, 1:1,000], anti-MPC2 [Proteintech, 20049-1-AP, 1:2000], anti-GAPDH [Cell Signaling, D4C6R-97166, 1:5,000]) overnight at 4 C, followed by HRP-conjugated secondary antibodies (Santa Cruz, sc-2354, 1:5,000) for 1 h at RT