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Despite minor discrepancies in research findings, the preponderance of evidence underscores coffees potential as a functional food with profound implications for healthspan and aging

For tumour xenograft models, 1 10 6 HCC-1806 cells or 5 10 6 PC3 cells were injected into the right fourth mammary fat pad or hind flank of athymic nude NU/J mice (The Jackson Laboratory, 002019) in 100 l sterile PBS or a 1:1 mixture of cell suspension in PBS and Matrigel (Corning, 356237) using a 29-gauge 1 ml insulin syringe

PvH was involved in the interpretation of the data and critical revision of the manuscript

Sherman S MM, Ungureanu, Rey JA