semaglutide biochemistry SEMAGLUTIDE - ANALYSIS

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The preparation method according to any one of claims 1 to 3, wherein the coupling in the fragmental synthesis of the Lys 26 side chain is performed by condensing with DIC+A or B+A+C until the resin is detected to be transparent with ninhydrin, wherein A is chosen from HOBt or HOAt, B is chosen from HBTU, HATU, TBTU, PyAOP, or PyBOP, C is chosen from DIPEA or TMP