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Preparation of the muscle specimens and frozen section To prepare frozen muscle sections, we first anesthetized adult fish with a 0.025% buffered MS-222 solution (Sigma-Aldrich, US), and then removed a segment of muscle tissue located between the anal and caudal fins 35

The central melanocortin system and physiological regulation

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No statistically significant differences in the concentrations of NOX1 and NCF2 were found between NETs-positive women and the control group